Apoptosis and Lipolysis of White Adipocytes Induced by Neuropeptide Y-Y5 Receptor Antisense Oligodeoxynucleotides

摘要

Objective: To investigate the influence of central administration of neuropeptide Y-Y5 receptor antisense oligodeoxynucleotides (ODNs) on body weight, fat pads of SD rats, and the effects of white adipocytes lipolysis and apoptosis. Methods: Y5 receptor antisense, sense, mismatched ODNs or vehicle was intracerebroventricularly (i. c. v.) injected. Average adipocyte area was calculated. DNA ladders were measured to evaluate adipocyte apoptosis, and RT-PCR was used to analyse the expression of Bcl-2 and Bax gene. Results: Central administration of Y5 antisense ODNs significantly decreased body weight, and average adipocyte area. DNA fragmentation was present after electrophoresis at epididymal adipose tissue. The expression of Bcl-2 gene was downregulated, while the expression of Bax upregulated. Conclusion: Lipolysis and adipocyte apoptosis may be important mechanisms far 75 antisense therapy.