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Role of transcription factor Egr-1 in liver injury following hemorrhagic shock and resuscitation

机译:转录因子Egr-1在失血性休克和复苏后肝损伤中的作用

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Objective: To investigate the role of transcription factor Egr-1 in liver injury following hemorrhagic shock (HS) /resuscitation (R). Methods: Both Egr-1 knockout (KO) and wild-type (WT) mice were subjected to HS and HSR injuries. The expressions of TNF-α, IL-6, G-CSF and ICAM-1 mRNAs in the liver were examined by RT-PCR, and their serum levels were measured by ELISA. The liver inflammatory infiltration and liver injury in both Egr-1 WT and KO mice following HS/R were evaluated by liver MPO content, serum ALT level and histological examination. Results : Egr-1 inhibition resulted in less mRNA expression of TNF-α, IL-6 , G-CSF and ICAM-1 in the liver, and lower serum levels of TNF-α, IL-6, G-CSF and ICAM-1 antigens in Egr-1 KO mice following HS/R. The liver inflammatory infiltration and liver injury were less severe in Egr-1 KO mice following HS/R, as evidenced by lower serum ALT level, lower hepatic MPO content and histological manifestations. Conclusion: Our data suggest that transcription factor Egr-1 is involved in regulating the expression of inflammatory response genes and plays a role in liver injury following HS/R.
机译:目的:探讨转录因子Egr-1在失血性休克(HS)/复苏(R)后肝损伤中的作用。方法:敲除Egr-1(KO)和野生型(WT)小鼠均遭受HS和HSR损伤。用RT-PCR检测肝脏中TNF-α,IL-6,G-CSF和ICAM-1 mRNA的表达,并用ELISA法测定其血清水平。通过肝脏MPO含量,血清ALT水平和组织学检查评估Egr-1 WT和KO小鼠在HS / R后的肝炎浸润和肝损伤。结果:Egr-1抑制导致肝脏中TNF-α,IL-6,G-CSF和ICAM-1的mRNA表达减少,血清TNF-α,IL-6,G-CSF和ICAM- HS / R后,Egr-1 KO小鼠中有1个抗原。 HS / R后,Egr-1 KO小鼠的肝脏炎性浸润和肝损伤较轻,这由较低的血清ALT水平,较低的肝MPO含量和组织学表现所证明。结论:我们的数据表明,转录因子Egr-1参与调节炎症反应基因的表达,并在HS / R后肝损伤中起作用。

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