In vitro biocompatibility evaluations of hyperbranched polyglycerol hybrid nanostructure as a candidate for nanomedicine applications

摘要

In the present study, a detailed biocompatibility testing of a novel class of hybrid nanostructure based on hyperbranched polyglycerol and β-cyclodextrin is conducted. This highly water soluble nanostructure with size of less than 10 nm, polydispersity of less than 1.3, chemical tenability and highly branched architecture with the control over branching structure could be potentially used as a carrier in drug delivery systems. To this end, extensive studies in vitro and in vivo conditions have to be demonstrated. The in vitro studies include in vitro cytotoxicity tests; MTT and Neutral Red assay as an indicator of mitochondrial and lysosomal function, and blood biocompatibility tests such as effects on coagulation cascade, and complement activation. The results show that these hybrid nanostructures, which can be prepared in a simple reaction, are considerably biocompatible. The in vivo studies showed that the hybrid nanostructure is well tolerated by rats even in high doses of 10 mg ml~(-1). After autopsy, the normal structure of liver tissue was observed; which divulges high biocompatibility and their potential applications as drug delivery and nanomedicine.