The Expression of Interleukin-17, Interferon-gamma, and Macrophage Inflammatory Protein-3 Alpha mRNA in Patients with Psoriasis Vulgaris

摘要

To investigate the role of Interleukin-17 (IL-17), Interferon-gamma (IFN-γ), and mac-rophage inflammatory protein-3 alpha (MIP-3α ) in the pathogenesis of psoriasis, reverse tran-scriptase-polymerase chain reaction (RT-PCR) was used to semi-quantitatively analyze the mRNA expression of IL-17, IFN-γ, and MIP-3α in 31 psoriatic lesions and 16 normal skin tissues. The results showed that the mRNA of the three cytokines was present in all specimens. And the expression level of IL-17 mRNA in skin lesions was 1.1416 + -0. 0591, which was significantly higher than that in normal controls (0. 8788 +- 0. 0344, P <0. 001). The expression levels of IFN-γ mRNA were 1. 1142 +- 0. 0561 and 0. 9050+-0. 0263, respectively, with significant difference(P <0. 001). And the expression levels of MIP-3α mRNA in psoriatic lesions was 1. 1397 +- 0. 0521, which was markedly higher than that in normal controls (0. 8681 +- 0. 0308, P <0. 001). These findings indicate that up-regulated expression of IL-17, IFN-γ, and MIP-3α might be involved in the pathogenesis of psoriasis.