Transmembrane Mass Transfer in Carrot Protoplasts during Osmotic Treatment

摘要

Minimized experiments with Confocal Scanning Laser Microscopy were used to describe mass transfer of isolated carrot protoplasts from at the usual conditions of the Osmotic Treatments (OT). Carrot protoplasts during OT with 30, 40 and 50% sucrose solutions were monitored. The ratio of cellular volume before and after OT with 30, 40 and 50% sucrose solutions was 0.86+- 0.12, 0.41+- 0.04 and 0.17+-0.02, respectively. Trans-membrane water flux was determined from cellular shrinkage, and the coefficient for water membrane permeability was (5.2+- 0.9) 10~(-6) mol~2/Jm~2s. To describe water transport in protoplasts at transient conditions, the diffusional approach was used. The effective water diffusivity during OT with 50% sucrose solutions was in the (0.8-1.8) 10~(12) m~2/s range.