Rapid and Simple Estimation of Microbiological Quality of Raw Milk Using Chromogenic Limulus Amoebocyte Lysate Endpoint Assay

摘要

A rapid chromogenic Limulus amoebocyte lysate (LAL) endpoint assay for the enumeration of total mesophilic microbial loads and coliforms was investigated as a means to assess the microbiological quality of raw milk. For experiment 1, raw milk samples (n=25) were stored in a refrigerator (2±2 deg. C) and then analyzed at regular intervals (1,5,10, and 15 days). For experiment 2, fresh raw milk samples (n=50) were tested to determine the utility of the LAL assay for fresh raw milk. The sample was diluted threefold in a 96-well microtiter plate with pyrogen-free water and assayed with a chromogenic LAL Kit to find a final reaction point.