STABILISED HYPOCHLOROUS ACID: A NEW THERAPEUTIC STRATEGY AGAINST DANGEROUS PARASITIC EYE INFECTION AGENT Acanthamoeba spp.

摘要

Acanthamoeba species are protozoan parasites that cause serious diseases in human. It is significant to investigate new treatment agents to combat the parasite which is very difficult to treat. The recent studies about stabilised hypochlorous acid indicated that with a rapid killing effect on different types of microorganisms, it could be used as a treatment agent for Acanthamoeba infections. Thus, we aimed to investigate the effect of stabilised hypochlorous acid on Acanthamoeba. Stabilised hypochlorous acid at serial dilutions of 1/2, 1/4, 1/8, 1/16, 1/32, and 1/64 was used in different time course. The direct microscopy for time-kill assay and Trypan blue assay were used to determine the effective dilutions and time exposure of stabilised hypochlorous acid to live form of parasite. We determined that the effect of stabilised hypochlorous acid was reached at 96 and 84% (dilution of 1/2 and 1/4, respectively) at the end of 90 min. The effect of stabilised hypochlorous acid was dose- and time-dependent. Stabilised hypochlorous acid solution could be used for eye infections due to the its safety feature. Since its effective feature at 1/2 and 1/4 dilutions within 0-10 min, it could be a new treatment agent to combat the parasite and its infections.