Evaluation of the effectiveness of land reclamation based on microbiological and biochemical parameters assessed in an ozokerite mining and processing landfill sown with Trifolium hybridum and Dactylis glomerata

摘要

The aim of the study was to analyse the microbiological and biochemical activity of a reclaimed landfill produced during extraction and processing ozokerite and sown with Trifolium hybridum and Dactylis glomerata. The analyses were carried out in the third year after application of two doses of sewage sludge, spent mushroom substrate, sawdust, and manure to the landfill soil. The following parameters were determined: the total number of oligotrophic bacteria and filamentous fungi, the number of cellulolytic and lipolytic bacteria and fungi, respiratory activity, the activity of dehydrogenases, beta-glucosidase, and lipase, and the activity of fluorescein diacetate hydrolysis. The results indicated that the wastes exerted a generally positive effect on the microbiological, biochemical, and enzymatic activities analysed in the reclaimed ground sown with both Trifolium hybridum and Dactylis glomerata. There was a negative effect on the total number of bacteria with low nutritional requirements, the number of cellulolytic bacteria, the number of lipolytic fungi, and the beta-glucosidase and lipase activities only in some objects. The strength of the effects was dependent on the type and dose of the waste and on the plant species. The strongest impact on the analysed parameters was exerted by the manure, sewage sludge, and spent mushroom substrate, whereas the addition of sawdust was found to be the least beneficial. The higher dose of manure and the lower dose of spent mushroom substrate were the most beneficial doses of the wastes. The number of lipolytic bacteria and cellulolytic bacteria and fungi, as well as the fluorescein hydrolytic activity followed by the activity of dehydrogenases, beta-glucosidase, and lipase were the most sensitive parameters.