A comparative study on decolorization of reactive azo and indigoid dyes by free/immobilized pellets of Trametes versicolor and Funalia trogii

摘要

The objective of the present study was to investigate decolorization of Acid Blue 74 and Reactive Blue 198 dyes by free and immobilized white rot fungal pellets in order to confirm the possibility of practical application via repeated-batch cultivation. Decolorization studies were conducted using free pellets (FP), fungal cells immobilized on activated carbon (IFCAC) and pinewood (IFCP), and also fungal cells entrapped in alginate beads (FCEAB). No additional nitrogen and carbon source was used and high decolorization rates were achieved in only dye-contained media without pH adjustment. Acid Blue 74 was decolorized 96 and 94% within 2 hr by Trametes versicolor and Funalia trogii free pellets, respectively. These values were 87 and 84% for Reactive Blue 198, in this respect. Immobilization of fungal cells on pinewood increased the usability of pellets and the average decolorization efficiency of both dyes. The micro environment changed in the presence of pinewood and increased the stability of immobilized pellets. Decolorization was performed rapidly and efficiently. Laccase activity enhanced with availability of pinewood, and high laccase production with F trogii was obtained. After separation by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), the molecular weight of Trametes versicolor and F trogii laccase bands was determined 64 and 61 kDa approximately. Green bands were obtained by the activity staining process with laccase substrate (ABTS) after gel renaturation step.