Changes in Enzyme Activity during Gibberellic Acid-Mediated Callus Growth in Dwarf Rice

摘要

Using the callus tissues of Tan ginbozu (dwarf) and Aichi asahi (normal), sequential changes in the growth patterns and the activities of some enzymes during aseptic culture were studied. (1) The control tissue of Aichi asahi began rapid growth after one week of calls culture, and this growth rate continued during the culture periods. In Tan ginbozu callus tissues, the sluggish growth rate continued during the culture periods. But GA3-response in Tan ginbozu became significant after 15 to 20 days of callus culture. The growth patterns of Tan ginbozu which displayed the GA3-response looked like that of control tissue of Aichi asahi. Protein content increased gradually after innnoculation of callus tissue, and reached its maximum values at 15 day in Tan ginbozu and 25 day in Aichi asahi. (2) In control tissue of Tan ginbozu and Aichi asahi, the activities of α-amylase, RNase, and acid phosphatase were minimum at 15 day and 8 day, respectively. However, it was observed that the minimum stages of α-amylase and RNase specific activity in Tan, ginbozu callus tissue responding to GA3 coincided with the patterns of enzyme activities in control tissue of Aichi asahi.