Lipolysis of Butter Oil by lmmobilized Lamb Pregastric Esterase: l. Uniresponse Kinetics--pH and Temperature Effects

摘要

Lamb pregastric esterase, immobilized by physical adsorption on microporous polypropylene in a hollow fiber reactor, has been employed to effect the continuous hydrolysis of the triglycerides in butter oil. Experimen- tal data were obtained at temperatures from 35 to 45 deg. C and pH values from 5.5 to 6.5. The overall rate of hydro- lysis was fastest at 40 deg. C and a pH of 6.0. Nonlinear regression methods were employed to determine the kinetic parameters of rate expressions based on a ge- neric Ping-Pong Bi Bi mechanism. The best nonlinear fit of the data was consistent with a mechanism that assumes that acylation of the enzyme is the rate-lim- iting step in the hydro1ysis reaction.