LactObacillus crispalus and its Nonaggregating Mutant in Human Colonization Trials

摘要

A wild-type Lactobacillus crispatus, showing a cel1 aggregation phenotype and its spontaneous nonaggre- gating mutant were compared for their in vitro adhe- sion properties to human ileal mucus and to a cultured human colonic cell line (Caco2) and for their in vivo colonization and adhesion potential with colonoscopy patients as volunteers in feeding trials. The wild-type strain adhered better to mucus or to Caco2 cells than did the mutant. Altogether, three human trials with the wild type and two with the mutant strain were performed. In two of the trials, the wild type could be recovered from either fecal samples or biopsies taken from the colon, while the mutant strain could not be demonstrated in either of the trials where it was used. The L. crispatus colonies recovered from the trials were often mixed, and several enterococci and lactobacillus strains coaggregating with L. crispatus wild type could be isolated. The results indicate that the surface-medi- ated properties, such as aggregation, of lactobacilli can have a role in adhesion and colonization.