Comparison of immunochromatographic assays based on fluorescent microsphere and quantum-dot submicrobead for quantitative detection of aflatoxin M_1 in milk

摘要

The performance of fluorescent microsphere immu-nochromatographic assay (FM-ICA) and quantum-dot submicrobead immunochromatographic assay (QB-ICA) was systematically and comprehensively com-pared in quantitative detection of aflatoxin M_1 in milk. Under optimum conditions, the advantages of FM-ICA include lower limit of detection of 42.3 pg/mL with bet-ter accuracy, precision, reliability, and practicability. The advantages of QB-ICA include shorter detection time and lower monoclonal antibody consumption. The 2 ICA were consistent with liquid chromatography-tandem mass spectrometry. This study serves as a reference for selecting appropriate fluorescent labels for the immunochromatographic assay of aflatoxin M_1.