Determination of the animal origin of raw food by species-specific PCR

摘要

Specific PCR, amplifying a fragment of the mitochondrially encoded cytochrome b gene, are developed for discriminatin of chicken, turkey, pig, cow and sheep. These PCR tests could be applied for detection and discrimination of animal food ingredients. For origin identification of milk, the bovine PCR is applied on DNA extracted with the Dneasy Tissue kit.