Crystallization of bFGF-DNA aptamer complexes complexes using a Sparse Matrix designed for protein-nucleic acid complexes

摘要

The Sparse Matrix approach for obtaining lead crystallization conditions has proven to be very fruitful for the crystallization of proteins and nucleic acids. Here we report a Sparse Matrix developed specifically for the crystallization of protein-DNA complexes. This method is rapid and economical, typically requiring 2.5 mg of complex to test 48 conditions.