Flow cytometric immunophenotyping of serous effusions and peritoneal washings: comparison with immunoctyochemsitry and morphological findings

摘要

Aim-To evaluate immunophenotyping by means of flow cytometry as a complemen- tary method for the detection of malig- nant cells in serous effusions and peritoneal washings. Material and methods-Frozen samples of 49 fresh serous effusions and peritoneal washings were analysed by flow cytometry, using monoclonal antibodies against CD45, Ber-EP4, and N-cadherin. Results were Compared with smear and cell block mor- Phology, as well as immunocytochemistry On paraffin wax embedded cell blocks.