Estimation of the taurocholate to lecithin molar ratio of mixed micelles in taurocholate-lecithin solutions by an interaction model and laser light-scattering measurements

摘要

The taurocholate (TC) to lecithin (L) molar ratio (K) and molecular weight of mixed TC-L micelles coexisting with simple TC micelles were determined by employing laser light-scattering measurement and an interaction model of mixed and simple micelles. When the nonmixed micellar taurocholate (NMTC) concentration was greater than 25 mM, the K value and molecular weight of mixed TC-L micelles were similar to 4.3 and 20,000 daltons, respectively, at an L concentration of 6 mM, and similar to 2.0 and similar to 35,000 daltons at L concentrations of 12 mM and 21 mM. A quantitative model of the formation of simple TC micelles and mixed TC-L micelles indicated that, when the NMTC concentration was greater than 25 mM, mixed TC-L micelles were unstable at low L levels because of low concentrations of L monomers. [References: 26]