首页> 外文期刊>Journal of the Chinese Chemical Society >DNA hydrolysis catalyzed by tris(diisopropyl-1,4,7-triazacyclononanes)ethane metal complexes
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DNA hydrolysis catalyzed by tris(diisopropyl-1,4,7-triazacyclononanes)ethane metal complexes

机译:三(二异丙基-1,4,7-三氮杂环壬烷)乙烷金属配合物催化的DNA水解

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摘要

Novel trinuclear coordinated ligands were designed using three of 1,4,7-triazacyclononane moieties with a tribromo linker. These ligands show greater capability and potency in association with various metal ions. Tris(diisopropyl-1,4,7-triazacyclononanes)ethane (2) associated with Cu2+ and Ce4+ ions shows better reactivity to achieve DNA hydrolysis at 37 degreesC at pH 8 for 5 hr. Using high resolution of polyacrylamide gel electrophoresis, DNA cleavage reaction in Tris-HCl buffer causes a nicking site at the single-stranded region. Alternatively, the reaction in HEPES buffer shows a random DNA cleavage included the double-stranded region. Hydrolysis of phosphodiester bond mediated by trinuclear metal complex is capable of performing at the physiological temperature 37 degreesC, but it has a lower reactivity compared to mono- and di-nuclear metal complexes. The result suggests that trinuclear metal complexes have a bulky environment to prevent from going toward the phosphodiester bond. The design of an efficient chemical hydrolyase will be a challenge in order to construct a coordinate ligand with the enhancement of hydrolytic activity and the reduction of the steric environment.
机译:使用具有三溴连接基的1,4,7-三氮杂环壬烷部分中的三个设计了新型三核配位体。这些配体与各种金属离子结合显示出更高的能力和效力。与Cu2 +和Ce4 +离子缔合的三(二异丙基-1,4,7-三氮杂环壬烷)乙烷(2)在37℃,pH 8的条件下进行5小时水解显示出更好的反应性。使用高分辨率的聚丙烯酰胺凝胶电泳,在Tris-HCl缓冲液中进行的DNA裂解反应会在单链区域产生切口部位。备选地,在HEPES缓冲液中的反应显示包括双链区的随机DNA切割。由三核金属配合物介导的磷酸二酯键的水解能够在37℃的生理温度下进行,但与单核和双核金属配合物相比,其反应性较低。结果表明,三核金属配合物具有庞大的环境,以防止其走向磷酸二酯键。为了构建具有增强水解活性和减少空间环境的配位体,有效的化学水解酶的设计将是一个挑战。

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