Myoglobin Detection: Utilizing Functional Magnetic Hybrid and Silica Micro Particles and Simple Molding Based Micro Channel Fabrication

摘要

A magnetic separator attached in a zig zag flow channel assembly has been fabricated to detect myoglobin on the micro particles. An anti-myoglobin conjugated magnetic hybrid and myoglobin conjugated silica micro particles were prepared and used for myoglobin detection on channel. For the detections, the competitive assay principle was followed based on affinity interaction of anti-myoglobin-myoglobin. The caliberation curve was plotted based on the deposit percentage of myoglobin conjugated silica micro particles. The calibration curve of the myoglobin showed the linear range between 1 × 10~(-8) - 5 × 10~(-11) (M) (R~2 = 0.9944). The minimum detectable concentration was 300 pg/mL. Hence all that concludes that, the application of zig zag flow channel with magnetic separator has offered new, simple and rapid approach for detecting myoglobin in whole blood samples with in 30 min.