Linking Diversity in Evolutionary Origin and Stereospecificity for Enoyl Thioester Reductases

摘要

The stereochemical course of reaction of crotonyl CoA by the novel crotonyl CoA reductase (CCR) of Streptomyces collinus from the pro-4S position of NADPH to the Re face of the β-carbon of crotonyl CoA. This transfer represents the first exception to the observation that enoyl thioester reductases catalyze transfer of the pro-2S hydrogen of NADPH to the Si face of the substrate.