Development of an All-Fluoroplastic Microfluidic Device Applied as a Nanoliter Sample Injector

摘要

A method was developed to fabricate an all-fluoroplastic microfluidic device using NEOFLON EFEP, a new fluoroplastic material. The device was successfully used as a nanoliter sample injector, which is very useful for various biological assays and chemical syntheses. The advantages of NEOFLON are that it is easily structured, it bonds at low temperatures, and it has chemical/physical robustness. NEOFLON microchannels 5 μm in width and 5μm in height were successfully fabricated for the prototype sample injector. The injector enabled the sample injection and metering of a 1.9nL sample with less than 2% volume change. This suggests that the NEOFLON flow device is capable of the precise fluidic handling of a few nanoliter samples in volume.