Analysis of metabolic pools in broilers chicks

摘要

This paper shows the possibility of obtaining new parameters for the mathematical modelling of data on stable isotopes in biological systems and its application in obtaining data on metabolic pools of blood plasma, blood serum, liver and muscle of broilers. This theory states that the modelling of turnover used for studies of isotopic incorporation when the metabolism has a single metabolic pool is feasible by the technique of setting an exponential. However, when the metabolism has more than one metabolic pool, it is necessary to apply the linearization technique, linear regression adjustment and evaluation of the assumptions of regression to obtain the kinetic parameters such as half-life (T-1/2) and isotope exchange rate (k). The application of this technique on carbon-13 data from 100 one-day-old chicks, with the change of diet composed of grains of the photosynthetic cycle of plants from C-4 to C-3, in broilers has enabled the discovery that the liver, blood plasma and blood serum have a single metabolic pool; however, the pectoral muscle has two metabolic pools. For the liver, blood plasma and blood serum, the half-life values were found by the exponential fit being T-1/2=1.4 days with the rate of exchange of k=0.502, T-1/2=2.4 days with k=0.293 and T-1/2=2.0 days with k=0.348, respectively. For the pectoral muscle, after linearization, the half-life values were found for T-1/2(1)=1.7 and T-1/2(2)=3 days, with exchange rates of k(1)=0.405 and k(2)=0.235, representing approximately 66 and 34%, respectively.