Dopamine release induced by atypical antipsychotics in prefrontal cortex requires 5-HT_1A receptors but not 5-HT_2A receptors

摘要

Atypical antipsychotic drugs (APDs) increase dopamine (DA) release in prefrontal cortex (PFC), an effect probably mediated by the direct or indirect activation of the 5-HT_1A receptor (5-HT_1AR). Given the very low in-vitro affinity of most APDs for 5-HT_1ARs and the large co-expression of 5-HT_1ARs and 5-HT_2A receptors (5-HT_2ARs) in the PFC, this effect might result from the imbalance of 5-HT_1AR and 5-HT_2AR activation after blockade of these receptors by APDs, for which they show high affinity. Here we tested this hypothesis by examining the dependence of the APD-induced DA release in medial PFC (mPFC) on each receptor by using in-vivo microdialysis in wild-type (WT) and 5-HT_1AR and 5-HT_2AR knockout (KO) mice. Local APDs (clozapine, olanzapine, risperidone) administered by reverse dialysis induced a dose-dependent increase in mPFC DA output equally in WT and 5-HT_2AR KO mice whereas the DA increase was absent in 5-HT_1AR KO mice. To examine the relative contribution of both receptors to the clozapine-induced DA release in rat mPFC, we silenced G-protein-coupled receptors (GPCRs) in vivo with N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ) while 5-HT_1ARs or 5-HT_2A/2CRs in the mPFC were selectively protected with the respective antagonists WAY-100635 or ritanserin. The inactivation of GPCRs while preserving ∼70% of 5-HT_2A/2CRs prevented the clozapine-induced DA rise in mPFC. In contrast, clozapine increased DA in mPFC of EEDQ-treated rats whose 5-HT_1ARs were protected (∼50% of control rats). These results indicate that (1) 5-HT_1ARs are necessary for the APDs-induced elevation in cortical DA transmission, and (2) this effect does not require 5-HT_2AR blockade by APDs.