Corona discharge ion mobility spectrometry for the determination of theophylline and guaifenesin in human serum

摘要

This paper describes the determination of theophylline and guaifenesin in human serum using ion mobility spectrometry with positive corona discharge as source ionization. The optimization of parameters that could influence ion mobility spectrometry was investigated. Under optimum conditions (Temperature; injection: 220 and oven: 175 °C, Flow rate; carrier: 300 and drift: 600 mL min−1, Voltage; corona: 2300 and drift: 7000 V, pulse width: 100 μs), calibration curves were linear in the ranges of 2 to120 and 6 to 120 ng for theophylline and guaifenesin, respectively. The relative standard deviation (n = 10) was below 12 %. The detection limits were found to be 0.1 ng for theophylline and 0.5 ng for guaifenesin. The recovery results for the theophylline and guaifenesin determination in human serum was about 80 % that indicate the proposed method can be applied for the two drugs analysis in real sample. Furthermore, the proposed method has been evaluated in the simultaneous determination of the two drugs.