Thermophilic dark fermentation of acid hydrolyzed waste ground wheat for hydrogen gas production

摘要

Batch dark fermentation experiments were performed to investigate the effects of biomass and substrate concentration on bio-hydrogen production from acid hydrolyzed ground wheat at 55 ℃. In the first set of experiments, the substrate concentration was constant at 20 g total sugar L~(-1) and biomass concentration was varied between 0.52 and 2.58 g L~(-1). Total sugar concentration was varied between 4.2 and 23.7 g L~(-1) in the second set of experiments with a 1.5 g L~(-1) constant biomass concentration. The highest cumulative hydrogen formation (582 mL, 30 ℃, 1 atm), formation rate (5.43 mL h~(-1)) and final total volatile fatty acid (TVFA) concentration (6.54 g L~(-1)) were obtained with 1.32 g IT1 biomass concentration. In variable substrate concentration experiments, the highest cumulative hydrogen (365 mL) and TVFA concentration (4.8 g L~(-1)) were obtained with 19.25 g L~(-1) initial total sugar concentration while hydrogen gas formation rate (12.95 mL h~(-1)) and the yield (200 mL H_2 g~(-1) total sugar) were the highest with 4.2 g L~(-1) total sugar concentration.