Optimization Of Cellulase Production By Bacillus cereus Through Response Surface Methodology And Its Application In Saccharification Of Biomass

摘要

In the present study. Bacillus cereus was isolated from decaying wood sample collected from Western Ghats of Karnataka for cellulase production and its gene sequence was deposited in gene bank (Bacillus cereus MH590292). The basal media (Czapek Dox) was optimized with respect to pH, temperature, carbon (Typha latifolia) and nitrogen (yeast extract) source using response surface methodology (RSM). Maximum Fpase production (exoglucanase) was recorded at pH 7.0, 45℃ temperature, 9% carbon and 0.7% nitrogen source. Crude cellulase enzyme from Bacillus cereus had effectively saccharified biomass into reducing sugar which shows that cellulase production from this strain using cheap lignocellulosic material is economically feasible for bioethanol production.