有機-無機ハイブリッド超薄膜によるバイオセンサの開発

摘要

カチオン性長鎖アルキル分子ODTAとPBナノクラスターrnから構成されるLB膜を酸化酵素溶液中に浸漬すると，静電rn相互作用により酵素がLB膜中に固定化されrnODTA／PB/Enzyme LB膜が得られる。このようにして作製さrnれた有機-無機ハイブリッドLB膜は，他物質の影響を排除rnした高精度なバイオセンサを構成する。%The hybrid ultra-thin films composed of cationic long-alkyl chain molecules and nano-size Prussian Blue clusters were obtained by the Langmuir-Blodgett (LB) technique. It was found that the LB films are quite useful as the starting material for building the high performance biosensors based on oxidative enzymes. The biosensors were easily prepared by a simple immersion of the LB films into enzyme solution, then they work under a very low potential range avoiding the interferent current. We demonstrated that the process is applicable to prepare many types of biosensors just by changing the immobilized enzymes.