Cell Growth, Cell-Cycle Progress, and Antibody Production in Hybridoma Cells Cultivated Under Mild Hypothermic Conditions

摘要

The effect of mild hypothermic (32°C) conditions on cell growth, cell-cycle progress, and antibody productionnof hybridoma C2E7 cells was investigated in the present study. The growth of hybridoma cells was slowernduring the mild hypothermic condition compared to that at 37°C; this led to about 10% decrease in maximumnviable cell density and volumetric antibody productivity. However, under mild hypothermic growth conditions,nthe culture viability was substantially improved and the specific antibody productivity was enhancedncompared to that at 37°C. The average specific productivity for the entire batch culture at 32°C is about 5%nhigher than that at 37°C. Cell-cycle analysis data showed that there was no growth arrestment during thenmild hypothermic growth of hybridoma cells. The G1-phase cells were increased, while the S-phase cells werendecreased gradually as the culture time progressed. Further analysis showed that the specific antibody productivitynof hybridoma cells was correlated to the fraction of S-phase cells.