Polycyclic aromatic hydrocarbon residues in blood serum and human milk in Egypt, A pilot case study

摘要

This study was performed to monitor residues of polycyclic aromatic hydrocarbons (PAHs) in samples of human blood serum and human milk taken from volunteers from one rural area of Egypt. Extraction and cleanup processes were conducted using the Quick Easy Cheap Effective Safe methodology. PAH residue analysis was performed by Gas chromatography-flame ioninzation detector (GC-FID) and High performance liquid chromatography-fluorescence detector (HPLC-FLD) for blood and milk samples, respectively. Some confirmatory work was conducted using a mass spectrometer. The concentrations of PAH residues in blood samples were between 0.007 and 0.407 mg/l, with many of the congeners below detection limits. Residues of the most carcinogenic PAH congeners including benzo (a) pyrene were below the limits of detection in all blood samples, and total PAH concentrations have ranged between 0.156 and 3.61 mg/l. Regarding human milk samples, the sum of PAH concentrations ranged from 95.23 to 229.26 (mu g/kg f.w.) with a mean of 154.35 (mu g/kg f.w.). Benzo[a] pyrene was detected in concentrations ranging from 0.348 to 15.4, with a mean of 7.872 (mu g/kg f.w.). Acenaphthylene, dibenzo [a,h] anthracene, acenaphthene, and naphthalene were the most abundant congeners in milk samples. Results indicated that the sources of PAHs in blood serum and human milk are of pyrogenic and petrogenic origin, respectively.