Determination of lignin content of eucalyptus globulus Wood using FTIR Spectroscopy

摘要

FTIR spectroscopy was used to quantitatively determine lignin in Eucalyptus globulus wood. A total of 40 wood samples from a 9 year old provenance trial was divided into two independent sets for calibration and validation. Lignin contents, as determined by the acetyl bromide method, ranged from 23/100 to 34/100 0.d. extractive-free wood. Spectra were recorded with the KBr pellet technique and twelve peaks between 1800 cm~1 to 800 cm~1 used for calibration by linear regression. The best calibration fit (R~2=0.98) was obtained with the 1505 cm~1 peak from lignin and the 1157 cm~1 peak from poly- saccharides as a reference.