GENOTOXICITY AND OXIDATIVE STRESS INDUCED BY SOME BISMUTH COMPOUNDS IN HUMAN BLOOD CELLS IN VITRO

摘要

The comparative effects of some bismuth compounds on oxidative stress and genotoxicity were assessed in human blood cells in vitro. Tested doses were 0.75, 1.5, 3 and 5 μg/mL of bismuth trioxide (Bi_2O_3), triphenyl bismuth (TPB), colloidal bismuth subcitrate (CBS), and bismuth subnitrate (BSN). The blood was processed to examine the following oxidative stress markers: glutathione (GSH), superoxide dismu-tase (SOD), catalase (CAT), and glucose-6-phosphate de-hydrogenase (G6PDH). In addition, the frequencies of sis-ter-chromatid exchanges (SCEs) in human peripheral lymphocytes were also determined by SCE test. The results show that treatments with bismuth compounds promote oxidative stress in human blood with an increase in concentrations. The biochemical changes observed in this tissue indicate that Bi_2O_3, TPB, CBS, and BSN (at the highest dose) act as pro-oxidant agents. Bismuth compound-induced changes in cellular parameters may be responsible for genotoxicity. On the concentration basis, the order of effectiveness in producing SCEs was: Bi_2O_3 > TPB > CBS > BSN.