ANALYSIS OF THE EFFECT OF BAF_1 MICROBIAL INOCULUM ON MICROBIOLOGICAL AND BIOCHEMICAL CONDITION OF PEAT UNDER SCARLET SAGE CULTIVATION

摘要

The objective of the performed experiments was to determine the dynamics of development of selected groups of microorganisms and the enzymatic activity in a substrate containing a microbiological inoculum (BAF_1) intended to improve scarlet sage growth and flowering. The material used in the investigations was peat substrate of 5.5-6.0 pH into which plants were planted and then inoculated with different doses of the BAF_1 biopreparation (1∶10, 1∶50, 1∶100) devised at the Department of General and Environmental Microbiology of Poznan University of Life Sciences. Samples of the substrate on which experimental plants were cultivated were collected during the following three phases: the phase of seedling planting, the phase of vegetative growth and the phase of flowering. The scope of investigations comprised determination of the developmental dynamics of total bacteria number, actinomycetes and molds using, for this purpose, the Koch plate method as well as levels of dehydrogenases, urease and acid phosphatase activity by the spectrophotometric method. In addition, the following plant morphological analyses were performed: plant height, shoot number and length, number of buds and inflorescences, content of chlorophyll a and b and leaf greenness index (SPAD). On the basis of the obtained research results, it was found that the applied BAF_1 biopreparation has a stimulating effect only on the development of the total number of bacteria and molds. Its inhibitory effect was recorded in case of actinomycetes and the activity of analysed enzymes. A positive effect of the BAF_1 inoculum was found on length and fresh weight of inflorescences in scarlet sage. No significant effect was found for the biopreparation on vegetative traits of plants. An exception in this respect was observed for fresh weight of shoots and leaves, which increased significantly under the influence of foliar application and broadcasting of the inoculum at a concentration of 1∶50 and 1∶100.