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Characteristics and Antioxidant Activity of Maillard Reaction Products from Fructose-glycine Oligomer

机译:果糖-甘氨酸低聚物的美拉德反应产物的特性和抗氧化活性

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The pH of Maillard reaction products (MRPs) derived from the Gly model system (Gly) decreased markedly as heating time increased. However the Digly model system (Digly) exhibited the highest increase in absorbance at 294 and 420 nm. Moreover, the loss of fructose and degree of sugar enolization in MRPs derived from the Trigly model system (Trigly) was the highest, whereas the glycine oligomer content in the Digly noticeably decreased as heating time increased. Furthermore, the gel permeation chromatograms (GPC) patterns of all MRP samples exhibited rising intensities as a function of the heating time, whereas the major peaks of each MRP sample were eluted at different retention times as glycine oligomer. Antioxidant activity of each MRP sample was investigated by Fe~(2+) and Cu~(2+) chelating activity, Trolox, ABTS, 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging activity, and ferric reducing ability of plasma (FRAP) assay. MRPs derived from the glycine oligomer were found to be effective antioxidants in different antioxidant activity assays.
机译:随着加热时间的增加,源自Gly模型系统(Gly)的美拉德反应产物(MRP)的pH值明显降低。但是,Digly模型系统(Digly)在294和420 nm处显示出最大的吸光度增加。此外,在源自Trigly模型系统(Trigly)的MRP中,果糖的损失和糖的烯化程度最高,而Digly中的甘氨酸低聚物含量则随着加热时间的增加而显着降低。此外,所有MRP样品的凝胶渗透色谱图(GPC)随加热时间呈上升趋势,而每个MRP样品的主峰在不同的保留时间被洗脱为甘氨酸低聚物。通过Fe〜(2+)和Cu〜(2+)的螯合活性,Trolox,ABTS,1,1-二苯基-2-吡咯-肼基(DPPH)自由基清除活性和三价铁还原反应来研究每个MRP样品的抗氧化活性。血浆(FRAP)分析能力。在不同的抗氧化剂活性测定中,发现甘氨酸低聚物衍生的MRP是有效的抗氧化剂。

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