Archaeal hyperthermostable mannitol dehydrogenases: A promising industrial enzymes for D-mannitol synthesis

摘要

Recently, the term healthy lifestyle connected to low-calorie diets, although it is not possible to get rid of added sugars as a source of energy, despite the close relation of added sugars to some diseases such as obesity, diabetes, etc. As a result, the sweetener market has flourished, which has led to increased demand for natural sweeteners such as polyols, including D-mannitol. Various methods have been developed to produce D-mannitol to achieve high productivity and low cost. In particular, metabolic engineering for D-mannitol considers one of the most promising approaches for D-mannitol production on the industrial scale. To date, the chemical process is not ideal for large-scale production because of its multistep mechanism involving hydrogenation and high cost. In this review, we highlight and present a comparative evaluation of the biochemical parameters that affecting Dmannitol synthesis from Thermotoga neapolitana and Thermotoga maritima mannitol dehydrogenase (MtDH) as a potential contribution for D-mannitol bio-synthesis. These species were selected because purified mannitol dehydrogenases from both strains have been reported to produce D-mannitol with no sorbitol formation under temperatures (90-120 degrees C).