Hydrogen sulflde synthesis in native Saccharomyces cerevisiae strains during alcoholic fermentations

摘要

AbstractIn order to diminish the undesirable impact of hydrogen sulfide (H2S) on wine, H2S synthesis was evaluated at phenotypic and transcriptional levels in 16 strains ofSaccharomyces cerevisiae, which comprised 12 natural isolates, three commercial strains, and one laboratory heterozygote. Strain-dependent and multi-gene participation traits were evident, and high gene activity did not necessarily elevated H2S levels. When the variation of gene expression was analyzed between fermentation stages in each strain, similarities among some strains related to H2S formation. UCD522 and seven strains with low H2S production were grouped together based on cluster analysis and fold-change analysis. They displayed a negative relationship between activity ofMET17,HOM2,SER33andCYS4and H2S formation, suggesting the role of biosynthesis of sulfur-containing amino acids. In the CECLFE1225, CECLFE1226 and UCD819 strains, transcriptional variation inMET3,MET5andMET10might account for the changes in H2S amount. High levels ofHOM2andSER33expression were implicated with the H2S phenotype of CECGM1 (H2S-free strain).MET1may be a key gene in sulfide biosynthesis owing to its involvement in almost all strains. This study furthers the understanding of H2S formation in differentS. cerevisiaestrains and the industrial application of natural isolates.Highlights•H2S synthesis in nativeS. cerevisiaestrains was tested for industrial interest.•H2S formation showed traits of strain-dependent and multi-gene participation.•Synthesis of S-containing amino acid affected H2S formation in seven native strains.•MET3,MET5andMET10might account for H2S amount changes in two native strains.