Biological Method for Determination of Residual Benzylpenicillin in Livestock Products

摘要

A biological method for determination of residual benzylpenicillin (PCG) in livestock products was evaluated. PCG was extracted from a sample with distilled water. The extract was deproteinized by adding tungstic acid, followed by a Bond Elut C18 cartridge clean-up procedure. The cylinder method was employed for quantitative determination, and microbioautography for identification of PCG. The average recovery at the maximum residue limits recommended by the Codex committee was 80.7% and the determination limits were 5 ppb (muscle, liver, kidney) and 1 ppb (milk).