Comparison of an HPTLC method with the Reflectoquant® assay for fast HMF determination in honey

摘要

5-Hydroxymethylfurfural (HMF) is a widespread process contaminant in carbohydrate-rich food and beverages. A re-cently developed high-performance thin-layer Chromatographie (HPTLC) method [1] was improved to obtain reliable findings for a wider ränge of honey matrices. After alcoholic extraction of the HMF from honey samples and application of the sample ex-tracts as 7 cm × 6 cm areas, a two-step development on HPTLC plates silica gel 60 was introduced. The first short development up to 2 cm with ethanol - methanol 9:1 (v/v) was essential for HMF release from the Start area. Then, the Separation up to 5 cm followed using ethyl acetate. Detection was performed densito- metrically by absorbance measurement at 290 nm.