Purification and detection of linamarin from cassava root cortex by high performance liquid chromatography

摘要

Linamarin, a cyanogenic glycoside in cassava (Manihot esculenta Crantz cv. KU-50) root cortex and parenchyma was extracted with different acids (HCl, H_2SO_4, H_3PO_4 or CH_3COOH) and detected by high performance liquid chromatography (HPLC). The highest linamarin was found in both tissues extracted with H_2SO_4. However, the concentration of linamarin in the extract of the root cortex was higher than that of the root parenchyma. Linamarin in crude extract of the root cortex using 0.25 M H_2SO_4 was purified by the second step HPLC with the yield of 91.54%. These extraction, detection and purification methods were useful to improve the purity of linamarin from cassava, especially the root cortex.