Analysis of 5-hydroxymethyl-2-furoic acid (HMFA) the main metabolite of alimentary 5-hydroxymethyl-2-furfural (HMF) with HPLC and GC in urine

摘要

A liquid chromatographic method with UV detection was developed to analyse 5-hydroxymethyl-2-fur-fural and its main metabolite 5-hydroxymethyl-2-furoic acid in urine. For the analysis on a reversed phase column was used with 5% methanol in water and 5 mM tetramethylammoniumhydrogen sulphate as ion-pair reagent as eluent. The detection was done at the UV absorption maximum of HMFA at 255 nm. The limit of quantification for HMFA was 7 mg/L with a recovery of 89%. For sample preparation the urine was centrifuged and the supernatant diluted with water. Three hundred samples of human urine were analysed. The concentration of HMFA was in the range of 0-100 mg/L with most of the samples around 10 mg/L.