Multivariate response surface methodology assisted modified QuEChERS extraction method for the evaluation of organophosphate pesticides in fruits and vegetables cultivated in Nilgiris, South India

摘要

An effective, simple and sensitive analytical method has been developed employing liquid chromatography coupled with tandem mass spectrometry and validated for estimation of five organophosphate pesticides at trace levels in six fruits and twelve vegetables. Plackett-Burman design and central composite design was used to screen and optimize the significant factors in modified QuEChERS (quick, easy, cheap, effective, rugged and safe) extraction method. The method evaluation was done by matrix-matched calibration with linearity ranging from 5 to 500 mu g/L with a correlation coefficient more than 0.990. The detection and quantification limit ranged from 0.1 to 1.0 mu g/kg and 0.5 to 5 mu g/kg, respectively. The mean recoveries were in the range of 76.89-110.30 % with the relative standard deviation less than 13.26% for all pesticides. Further, the method developed was applied to analyze real samples cultivated in the hill areas of Nilgiris, South India.