Development of a screening analytical method for the determination of non-dioxin-like polychlorinated biphenyls in chicken eggs by gas chromatography and electron capture detection

摘要

A sensitive and reproducible screening analytical method is here proposed for the determination of six non dioxin-like polychlorinated biphenyls (NDL-PCBs, congener 28, 52, 101, 138, 153, 180) in chicken eggs based on accelerated solvent extraction (ASE) procedure for the fat extraction and determination, a solid phase extraction (SPE) sample clean-up process, and a gas chromatography - electron capture detection (GC-ECD) analysis. The optimized chromatographic separation, in less than 25min, returned good responses for the six NDL-PCBs in the range of 2.5-60.0 mu g L-1, with correlation coefficients always higher than 0.9995. Instrumental limits of detection were between 0.08-0.35 mu g L-1, corresponding to 0.05 and 0.23ng g(-1) fat in the matrix, while method detection limits, calculated on spiked egg samples, ranged from 1.6 to 3.5ng g(-1) fat. The method has been extensively validated in terms of selectivity, sensitivity, recovery, precision, ruggedness and measurement uncertainty, following the European Directives.