2. BIOCHEMISTRY, MOLECULAR BIOLOGY AND BIOTECHNOLOGY

摘要

A new method for synthesizing the labeled 1-tryptophan is described in this work. 1-Tryptophan, labeled with 98% ~(15)N at position 1 was synthesized from the labeled anthranilic acid using Candida utilis mutants. The conversion ratio of ~(15)N of 50% was achieved. The labeled anthranilic acid was synthesized by [~(15)N] phthalimide that was prepared by 99.34% [~(15)N] urea and phthalic anhydride in ortho-xylene medium at 140℃ and under atmospheric pressure.