RAPD–2100 bio-analyzer and 2DGE methods applied to qualitatively and quantitatively assess grain purity of commercial malting barley

摘要

Grain purity is of great interest to malting barley trader and beer producer. DNA typing method has been applied for this purpose, but typically, single grain was tested and the procedure was expensive, laborious, and time-consuming. This study first reported fast semi-quantification of cereal seed by DNA test of blended sample. It was helpful for rapid screening of large amount of samples. Particularly, RAPD and 2100 bio-analyzer were combined to determine the purity range of malting barley grain taking advantage of mathematical linear relationship between concentration of marker bands and grain purity. Statistical analysis of 11 independent assays resulted in quantitative parameters for each RAPD marker band. Test of 14 samples using the fast method showed an average of 11% deviation to rated value. Protein profiling method helped to distinguish cultivars which were of identical RAPD pattern. Quantification by protein profile was also explored.