Anticancer Activity of Kalanchoe tubiflora Extract Against Human Lung Cancer Cells In Vitro and In Vivo

摘要

Uncontrolled cell proliferation is a common feature of human cancer. Some of herbal extract or plant-derived medicine had been shown as an important source of effective anticancer agents. We previ ously reported that an n-BuOH-soluble fraction of Kalanchoe tubiflora has antiproliferative activity by inducing mitotic catastrophe. In this study, we showed that the H_2O-soluble fraction of Kalanchoe tubi flora (KT-W) caused cell cycle arrest, and senescence-inducing activities in A549 cells. We used 2 dimen sional PAGE to analyze the protein expression levels after KT-W treatment, and identified that the energy metabolism-related proteins and senescence-related proteins were disturbed. In vivo experiments showed that the tumor growths in A549-xenografted nude mice were effectively inhibited by KT-W. Our findings implied that KT-W is a putative antitumor agent by inducing cell cycle arrest and senescence.