DERIVATION AND APPLICATION OF RELATIVE POTENCY ESTIMATES BASED ON IN VITRO BIOASSAY RESULTS

摘要

Relative potency(REP)estimates are widely used to characterize and compare the potency of a wide variety of samples analyzed using in vitro bioassay. Relative potency estimates are generally calculated as a simple ratio: the EC50 of a well- characterized standard divided by the EC50 of a sample. Such estimates are valid only when the dose-response curves for the sample and standard are parallel and exhibit the same maximum achievable response(efficacy). These conditions are often either violated or cannot be demonstrated.