Triclocarban-induced change in intracellular Ca~(2+) level in rat thymocytes: Cytometric analysis with Fluo-3 under Zn~(2+)-free conditions

摘要

Triclocarban (TCC) is an antimicrobial used in personal hygiene products. Recent health concerns arose after TCC was detected in the blood of human subjects who showered with soap containing TCC. In this study, the effect of TCC on intracellular Ca~(2+) concentration in rat thymocytes was examined using Fluo-3, an indicator of intracellular Ca~(2+). TCC at concentrations ranging from 0.1 μM to 3 μM increased intracellular Ca~(2+) concentration biphasically: first by releasing Ca~(2+) from intracellular Ca~(2+) stores and then inducing Ca~(2+) influx through store-operated Ca~(2+) channels. The threshold TCC concentration to increase intracellular Ca~(2+) concentration in this study was lower than the maximum TCC concentrations reported in human blood samples. Therefore, we anticipate that TCC at concentrations reported in human blood samples might disturb intracellular Ca~(2+) signaling in human lymphocytes.