Silver, zinc oxide and titanium dioxide nanoparticle ecotoxicity to bioluminescent Pseudomonas putida in laboratory medium and artificial wastewater

摘要

Bacteria based ecotoxicology assessment of manufactured nanoparticles is largely restricted to Escher-ichia coli bioreporters in laboratory media. Here, toxicity effects of model OECD nanoparticles (Ag NM-300K, ZnO NM-110 and TiO_2 NM-104) were assessed using the switch-off luminescent Pseudomonas putida BS566::luxCDABE bioreporter in Luria Bertani (LB) medium and artificial wastewater (AW). IC_(50) values ～4 mg L~(-1),100 mg L~(-1) and ＞200 mg L~(-1) at 1 h were observed in LB for Ag NM-300K, ZnO NM-110 and TiO_2 NM-104, respectively. Similar results were obtained in AW for Ag NM-300K (IC_(50) ～5 mg L~(-1)) and TiO_2 NM-104 (IC_(50) ＞200 mg L~(-1)) whereas ZnO NM-110 was significantly higher (IC_(50) ＞200 mg L~(-1)). Lower ZnO NM-110 toxicity in AW compared to LB was associated with differences in agglomeration status and dissolution rate. This work demonstrates the importance of nanoecotoxicological studies in environmentally relevant matrices.