Simultaneous determination of parathion and p-nitrophenol in vegetable tissues by derivative spectrophotometry

摘要

A first derivative spectrophotometric method has been developed for the determination of parathion and p-nitrophenol in vegetable tissues. Ethanol was used as solvent for extracting the compounds from the tissues and subsequently the samples were evaluated against a vegetable tissue blank, directly by derivative spectrophotometry. The simultaneous determination of these compounds can be carried out using the zero-crossing approach for parathion at 253.0 nm and for p-nitrophenol at 273.1 nm. In the samples each analyte was determined in the presence of one another in the ranges between 4.9 to 3883.5 μg g~-1 for parathion and 4.9 to 3285.3 μg g~-1 for p-nitrophenol.