Hydroxylated PCB metabolites (OH-PCBs) in archived serum from 1950-60s California mothers: A pilot study

摘要

We are studying participants selected from the Child Health and Development Studies (CHDS), a longitudinal birth cohort of over 20,000 California pregnancies between 1959 and 1967, for associations between maternal body burden of organochlorine contaminants and thyroid function. We designed a pilot study using 30 samples selected among samples with high and low PCB concentrations to evaluate the feasibility of measuring OH-PCBs in the larger study population. GC-ECD and GC-NCI/MS were used to determine PCBs and OH-PCBs as methyl derivatives, respectively. Maternal serum levels of ∑_(11)PCBs and ∑_8OH-PCB metabolites varied from 0.74 to 7.99 ng/mL wet wt. with a median of 3.05 ng/mL, and from 0.12 to 0.98 ng/mL wet wt. with a median of 0.39 ng/mL, respectively. Average concentrations of ∑_8OH-PCB metabolites in the high PCB group were significantly higher than those in the low PCB group (p<0.05). The levels of OH-PCB metabolites were dependent on PCB levels (r = 0.58, p<0.05) but approximately an order of magnitude lower (p<0.05). The average ratio of ∑_8OH-PCBs to ∑_(11)PCBs was 0.14±0.08. The primary metabolite was 4-OH-CB187 followed by 4-OH-CB107. Both of these metabolites interfere with the thyroid system in in vitro, animal, and human studies. OH-PCBs were detectable in all archived sera analyzed, supporting the feasibility to measure OH-PCB metabolites in the entire cohort.