Isolation and characterisation of genomic and cDNA clones for an androgen-regulated secretory protein of rat seminal vesicles

摘要

Testosterone controls the synthesis of seminal vesicle protein F in male rats by regulating the cellular concentration of its mRNA (mRNA_F). Phage λ recombinants have been isolated containing the complete F gene. In addition plasmids have been constructed containing cDNA_F sequences some of which are probably full-length (∽700 bp). Detailed restriction mapping shows that the F gene is 1.7 kbp long and contains ∽1.0 kbp of intervening sequencearranged in at least two introns (420 bp and 600 bp). Part of cDNA_F has been sequenced showing that the terminal125 bp of the 3' untranslated region of mHNA_F has substantial (70%) sequence homology with the 3'end of the mRNA coding for another androgen-dependent seminal vesicle protein (protein S). The cloned F gene has been detected in liver and seminal vesicle DNA along with an homologous but structurally different gene. The hormonal control of mRNA_F was examined with cDNA_F. A pronounced (∽3000-fold) differential response to testosterone was observed.